A composite video exhibiting intensities of polarized emission of a fluorophore attached to an AdiC protein molecule shown in Fig. 1, integrated intensity values, calculated angles, and electron densities of AdiC in different conformations. Shown on the left are the original recorded intensities I0, I45, I90, and I135 displayed from top to bottom; on the middle left are running traces of integrated intensity values with background subtracted; on the middle right are running traces of angles θ and φ along with Ω, where each event is color coded according to the state that AdiC adopts; C1 (yellow) corresponds to the EX conformation, C2 (blue) to EO, C3 (orange) to IO, and C4 (cyan) to IX; on the right is a video of the AdiC molecule transitioning among four conformational states represented by the electron densities of AdiC (PDBs 7O82 and 3L1L) and those of the related BasC and ApcT transporters proper (PDBs 6F2G and 3GIA), as described in Results. At a given time point, the electron density corresponding to the conformation identified by the polarization study is displayed. Due to the limit of allowable file size, the video was made to be viewed in a small window of such software as Windows Media Player.
