A CON myocyte cultured for 12 h under the control conditions and immunostained for nCadherin (blue, sheep anti-nCad/Alexa405 donkey anti-goat), NaV1.5 (green, rabbit anti-NaV1.5/Alexa488 donkey anti-rabbit), β-Tub (red, mouse anti-β-tubulin/Alexa568 donkey anti-mouse), and EB1 (white, rat anti-EB1/Alexa647 goat anti-rat). The video shows rotating 3-D views of the myocyte end in the following sequence: (a) merged four colors, (b) nCad alone, (c) nCad plus the ICD volume as a magenta semitransparent surface, (d) NaV1.5 with the ICD volume, (e) EB1 with the ICD volume, and (f) β-Tub with the ICD volume. The 3-D views and animation were created with IMARIS (v. 10).
