Confocal live-cell imaging of Jurkat cells transiently expressing GFP-tagged NFAT. In the resting state, GFP fluorescence is predominantly visible in the cytosol. 8 min after stimulation with 5 Gy x rays, NFAT-GFP fluorescence alters its location and progressively moves over a period of ∼30 min completely into the nucleus. Time in h:min; images taken with a frame rate of one/5 s.
