Video 2.

Representative video showing a region of interest that was tracked. White box shows the two QDs of different color attached to the same filament moving along actin that provided the data in Fig. 2. Field is 503 × 192 pixels, 1,500 frames acquired at 20-ms exposure time. Playback is set at 200 frames s−1. Each pixel is 86.7 × 86.7 nm. [ATP] = 10 µM.


Evidence for S2 flexibility by direct visualization of quantum dot–labeled myosin heads and rods within smooth muscle myosin filaments moving on actin in vitro

Richard K. Brizendine, Murali Anuganti, and Christine R. Cremo

DOI: 10.1085/jgp.202012751
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