Representative video of RLC-LMM cofilaments labeled with 605 nm QD-SpyC and 705 nm QD-SnoopC moving along actin filaments. 605 nm QD-SpyC (green) and 705 nm QD-SnoopC labeled (red) SMM filaments are seen moving on surface-attached actin filaments (blue). Field is 503 × 234 pixels, 1,500 frames acquired at 20-ms exposure time. Playback is set at 200 frames s−1. Each pixel is 86.7 × 86.7 nm. [ATP] = 10 µM.
