A link between the dynamics of the D447:W434 hydrogen-bond and D447:T449 hydrogen-bond and the water traffic through the upper barrier. The movie depicts the dynamics of the upper-barrier residues of a single WT channel subunit during a 100-ns MD run. Water molecules that come into a hydrogen-bond distance (<4 Å) from the side chain of D447 are shown. The time-series plots to the right depicts the diameter of the D-W gate (top), the D447:T449 distance (middle), and the water traffic (bottom).
