Examples of STICCS correlation function fluctuations over time. Each row shows the PIEZO1-GFP autocorrelation function (left), the CtxB–Alexa Fluor 555 autocorrelation function (center), and the cross-correlation function (right) evolution for each tested condition, over the course of a typical experiment (time expressed in frame time blocks τ, top right). Ctrl, untreated control; chol, water-soluble cholesterol/MBCD complex; dyno, dynasore.