Thermal activation of reconstituted thin filaments at pCa 9. Fluorescence images of reconstituted thin filaments on a rabbit fast skeletal HMM-coated coverslip were obtained. The IR laser was irradiated for 2 s, as indicated by “Heating.” Baseline temperature, 25 ± 1°C. Objective lens, 60× (N/A, 1.45; oil immersion). Speed, 33 fps. Scale bar, 20 µm. See Fig. 2.
