Ventricular myocyte in the beating heart in vivo with image sequence reconstruction. Cardiomyocyte expressing α-actinin–AcGFP located in the central part of the LV was imaged (see Figs. 6B and S4, A–D). Objective lens, 60× (N/A, 1.00; water immersion). Speed, 100 fps. Bar, 10 µm.