3D reconstruction of an astrocyte in an MNTB slice, a series of 35 two-photon images were taken in steps of 1 µm. The fluorescence is due to the expression of eGFP in the GFAP-eGFP transgenic mouse line. The reconstruction displays 51 frames at a speed of 6 frames per second, turning 360° in 7° steps. The video shows that the PPN, visible as a hollow area in the center, is completely covered by astrocytic processes and that the astrocyte soma is in close contact to the PPN. The diameter of the displayed frame is 90 µm. Note that the astrocyte is located on the PPN like a hat on a head.
