Live imaging of T cells, and T cells and B cells interacting in the adult zebrafish ALO. Ex vivo confocal micrograph extended depth-of-focus and maximum intensity projection time-lapse movies of the ALO of an adult Tg(lck:egfp)cz2 transgenic zebrafish with T cells labeled in green, followed by a time-lapse movie of T-cell Tg(lck:mcherry)ns107–B cell Tg(cd79b:egfp)fcc89 interactions.