Human NLRP1 is activated by the ribotoxic stress response. N/TERT-1C1C-EGFP cells in the presence of PI were treated with DMSO and recorded by wide-field microscopy over time. C1C-EGFP is displayed in green, PI signal in red, and phase contrast in grayscale. The time after stimulation is shown as hh:mm; the frame rate is 7 fps.