Endogenous CXCL13 maintains B cell margination. Related to Fig. 5. Intravital lung microscopy was used to investigate the role of endogenous CXCL13 on CD19+ B cell (Cd19ZsGreen1; green) margination in the lung capillaries. B cell margination was assessed 12 h after i.p. injection of either the isotype control antibody (left) or anti-CXCL13 neutralizing antibody (right). Experiments were repeated three times (n = 3). Playback speed is 12 frames/s, 10 min actual imaging time.
