Video 5.

CXCL13 enhances B cell margination in the lung. Related to Fig. 5. Intravital lung microscopy was used to investigate the role of exogenously provided CXCL13 on CD19+ B cell (Cd19ZsGreen1; green) margination in the lung capillaries. 12 h after i.t. CXCL13 injection, B cell marginated behavior was assessed. Ly6G+ neutrophils (fluorochrome-conjugated anti-Ly6G antibody; blue) were also labeled. Experiments were repeated three times (n = 3). Playback speed is 12 frames/s, 10 min actual imaging time.


Marginating transitional B cells modulate neutrophils in the lung during inflammation and pneumonia

John Podstawka, Sarthak Sinha, Carlos H. Hiroki, Nicole Sarden, Elise Granton, Elodie Labit, Jung Hwan Kim, Graciela Andonegui, Yuefei Lou, Brendan D. Snarr, Donald C. Sheppard, Nicole L. Rosin, Jeff Biernaskie, and Bryan G. Yipp

DOI: 10.1084/jem.20210409
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