IgD+ B cells marginate in the lung vasculature. Related to Fig. 4. Intravital lung microscopy was used to investigate if transitional B cells marginate in the lung vasculature. Cd19ZsGreen1 mice (white in video) were costained with anti-IgD fluorescently conjugated antibody (red). Experiments were repeated three times (n = 3). Playback speed is 24 frames/s, 10 min actual imaging time per segment.