B cells marginate in the lung capillaries. Related to Fig. 1. Intravital lung imaging was used to assess CD19+ B cell (Cd19ZsGreen1; green) and Ly6G+ neutrophil (fluorochrome-conjugated anti-Ly6G antibody; blue) marginated behavior in the lung capillaries. The red arrows point to examples of marginated B cell behavior. Transit time was quantified from the B cells and neutrophils present at time 0. Experiments were repeated three times (n = 3). Playback speed is 12 frames/s, 20 min actual imaging time.
