ENDS sliding and detaching from the venular wall in vivo. Mouse cremaster intravital microscopy record, two ENDS (examples 1 and 2) sliding and detaching from the wall of the same venule are shown. Rectangles indicate where the ENDS are. Direction of blood flow is from right to left. The mouse was intrascrotally preinjected with TNFα to stimulate neutrophil interaction with the vasculature. Neutrophils were labeled via intravenous injection of Ly6G-AF647 antibody (magenta). Original speed, 4.76 frame/s, exported at 8 frames/s; playback speed is 1.76 times faster than real speed.
