ENDS formation in vivo. Mouse cremaster intravital microscopy record, a rolling neutrophil leaves an ENDS behind on the venular wall. Direction of blood flow is from right to left. The mouse was intrascrotally preinjected with TNFα to stimulate neutrophil interaction with the vasculature. Neutrophils were labeled via intravenous injection of Ly6G-AF647 antibody (magenta). Original speed, 3.62 frames/s, exported at 10 frames/s; playback speed 2.76 times faster than real speed.
