Ce3D of the medLN and associated lymphatics of a mouse infected 16 d earlier with PR8-gp33. Isolated medLN and associated lymphatics were made optically transparent as previously described (Li et al., 2017). Cleared tissue was imaged by tiling Z-stacks with confocal laser scanning microscopy (Leica SP5). 3D reconstructions were done using Imaris (Bitplane) software v9.2.1, and GFP+ P14 cells were displayed through a surface mask. Green, GFP+ P14 CD8+ T cells; magenta, CD103; blue, Lyve1. Corresponding still images from this video are displayed in Fig. 8 E. Images are representative of three Ce3D-processed tissue specimens comprising the lung, medLN, and medLN-associated lymphatics.
