Large-scale 4D whole calvarial BM imaging. Representative video illustrating real-time interaction with 4D whole BM intravital time course data from Pf4-Cre:mTmG reporter mice using Fiji software. This video illustrates the ability to visualize a large number of Mks by moving in the x, y, and z axes of data more than ∼6 h of imaging in live adult mice. By taking this approach, the behavior of all Mks in the calvarial BM can be observed and quantified, allowing detailed analysis of individual Mks and the organ as a whole system. The cursor highlights movement within the individual dimensions of the dataset and an example of proPlt production by a single Mk. This video is representative of three individual biological replicate datasets. Data presented here has been downscaled in resolution for uploading purposes. Green, Mks/Plts; gray, bone (SHG); red, non-Mk cells/stroma. Original data = 1 frame (z-stack) every 3.5 min.
