Video 2.

Ex vivo proPlt formation is rare but can occur from Mks during E13.5 FL organotypic culture. Video depicts a proPlt forming Mk (gray) that undergoes the classic changes in volume and sphericity typical of the process. Initiation of proPlt formation is represented by the formation of a leading edge. Time shown as hours:minutes:seconds. Original data = 1 frame (z-stack) every 3 min.

Membrane budding is a major mechanism of in vivo platelet biogenesis

Kathryn S. Potts, Alison Farley, Caleb A. Dawson, Joel Rimes, Christine Biben, Carolyn de Graaf, Margaret A. Potts, Olivia J. Stonehouse, Amandine Carmagnac, Pradnya Gangatirkar, Emma C. Josefsson, Casey Anttila, Daniela Amann-Zalcenstein, Shalin Naik, Warren S. Alexander, Douglas J. Hilton, Edwin D. Hawkins, and Samir Taoudi

DOI: 10.1084/jem.20191206