Two-photon live imaging of a temporary contact ex vivo. Related to Fig. 2. Organotypic hippocampal slices from CX3CR1GFP pups were co-cultured with pathogenic B6.2D2.RFP.Th17 cells for 24 h and contacts visualized over a time period of 10 min in n = 4 organotypic slices from three different experiments. Shown here is the original GFP (myeloid cells) and RFP (Th17 cells) 3D image sequence (smoothened and 3D cropped in Imaris to show a temporary contact) as well as the 3D-reconstructed version (Imaris; green is myeloid cells, and red is Th17 cells). Time is shown in h/min/s.
