Video 17.

Combining AMR deficiency with MGL blockade significantly reduced the amount of platelets accumulating on Kupffer cells after sialidase injection. Time-lapse SD-IVM video of the liver of Asgr1^−/− mice treated with MGL1/2 blocking antibody and injected i.v. with 50 mU sialidase. Kupffer cells (blue) were stained using an anti-F4/80 antibody. Endogenous platelets (red) were labeled using an anti-CD49b antibody. Frame rate is 20 fps.

Macrophage galactose lectin is critical for Kupffer cells to clear aged platelets

Carsten Deppermann, Rachel M. Kratofil, Moritz Peiseler, Bruna A. David, Joel Zindel, Fernanda Vargas E Silva Castanheira, Fardau van der Wal, Agostina Carestia, Craig N. Jenne, Jamey D. Marth, and Paul Kubes

DOI: 10.1084/jem.20190723