Video 6.

Example of a CAR T cell forming prolonged interactions with tumor cells without inducing their apoptosis. C57BL/6 mice were sublethally irradiated and injected with Eμ-myc-DEVD tumor cells 7 d before treating them with GFP⁺ CAR T cells. Mice were subjected to intravital bone marrow imaging 40 h after CAR T cell transfer. Live tumor cells appear in gray, apoptotic tumor cells in blue, and T cells in green. Scale bar represents 20 μm. Total duration = 78 min.

Single-cell imaging of CAR T cell activity in vivo reveals extensive functional and anatomical heterogeneity

Marine Cazaux, Capucine L. Grandjean, Fabrice Lemaître, Zacarias Garcia, Richard J. Beck, Idan Milo, Jérémy Postat, Joost B. Beltman, Eleanor J. Cheadle, and Philippe Bousso

DOI: 10.1084/jem.20182375