Video 4.

Example of a B cell tumor undergoing apoptosis without apparent CAR T cell contacts. C57BL/6 mice were sub-lethally irradiated and injected with Eμ-myc-DEVD tumor cells 7 d before treating them with GFP⁺ CAR T cells. Mice were subjected to intravital bone marrow imaging 40 h after CAR T cell transfer. The square highlights a tumor cell undergoing apoptosis in the absence of apparent interactions with CAR T cells. Scale bar represents 20 μm. Total duration = 64 min.

Single-cell imaging of CAR T cell activity in vivo reveals extensive functional and anatomical heterogeneity

Marine Cazaux, Capucine L. Grandjean, Fabrice Lemaître, Zacarias Garcia, Richard J. Beck, Idan Milo, Jérémy Postat, Joost B. Beltman, Eleanor J. Cheadle, and Philippe Bousso

DOI: 10.1084/jem.20182375