Video 2.

Example of a 17-h time-lapse video showing a 350-μm-wide microwell, containing HeLa-CD48/NES-ELQTD-GFP-T2A-NES-VGPD-mCherry cells and four NK cells (not fluorescent). NK cell tracks are marked by different colors, and killing events are indicated by red crosses. The time resolution is 3 min between frames.

NK cells switch from granzyme B to death receptor–mediated cytotoxicity during serial killing

Isabel Prager, Clarissa Liesche, Hanna van Ooijen, Doris Urlaub, Quentin Verron, Niklas Sandström, Frank Fasbender, Maren Claus, Roland Eils, Joël Beaudouin, Björn Önfelt, and Carsten Watzl

DOI: 10.1084/jem.20181454