Long-lasting contacts with DCs are restricted to undivided T cells. GFP-expressing OT-I CD8+ T cells were labeled with SNARF and transferred into recipient mice. CFP-expressing DCs pulsed with OVAp were injected in the footpad. At 48 h, recipient mice were subjected to intravital imaging of the draining popliteal lymph node. Representative time-lapse video showing that, in the same lymph node, undivided (yellow) but not divided (green) T cells established long-lived interactions with DCs (cyan).
