Time-lapse imaging of endothelial-hematopoietic transition in the PBI of Tg(flk1:Dendra2) embryos (related to Fig. 3). Time-lapse confocal imaging shows a photoconverted endothelial cell (red) in the ventral wall of caudal aorta of Tg(flk1:Dendra2) embryo undergoes endothelial-hematopoietic transition to form a nascent hematopoietic progenitor, which subsequently divides into two daughter cells and migrate to the CVP niche. Time on the upper-left corner indicates the developmental stage of the embryo. Each frame was collected with 10-min interval, and video is displayed at 15 frames/s.
