S. pneumoniae movement in splenic MZ. MZ macrophages (CD209b; red) capture of i.v. injected S. pneumoniae (5 × 106 CFU D39-GFP) from the circulation in wild-type mice. Bacteria were injected 30 s before initiation of the 20-min time interval visualized by two-photon IVM. The playback rate is eight frames per second.
