Video 2.

Visualization of Ly6C^hi monocytes in Cx3cr1^gfp/+ mice with an anti-Ly6B.2 antibody. Time-lapse image sequence of maximum intensity projection displaying the behavior of GFP⁺ cells in the lungs (alveolar space) of Cx3cr1^gfp/+ mice. Evans blue (50 µg) was administered i.v. to delineate the lung vasculature. 5 min after the beginning of imaging, Ly6B.2-PE (4 µg) was injected i.v., and allowed the visualization of Ly6C^hi monocytes (Ly6B.2⁺CX₃CR1⁺), Ly6C^lo monocytes (Ly6B.2⁻CX₃CR1⁺) and neutrophils (Ly6B.2⁺CX₃CR1⁻). Video is representative of 3 mice. Elapsed time is shown as hours:minutes:seconds. Scale bar: 40 µm.

CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses

Shu Zhen Chong, Maximilien Evrard, Sapna Devi, Jinmiao Chen, Jyue Yuan Lim, Peter See, Yiru Zhang, José M. Adrover, Bernett Lee, Leonard Tan, Jackson L.Y. Li, Ka Hang Liong, Cindy Phua, Akhila Balachander, Adrian Boey, David Liebl, Suet Mien Tan, Jerry K.Y. Chan, Karl Balabanian, John E. Harris, Mariaelvy Bianchini, Christian Weber, Johan Duchene, Josephine Lum, Michael Poidinger, Qingfeng Chen, Laurent Rénia, Cheng-I Wang, Anis Larbi, Gwendalyn J. Randolph, Wolfgang Weninger, Mark R. Looney, Matthew F. Krummel, Subhra K. Biswas, Florent Ginhoux, Andrés Hidalgo, Françoise Bachelerie, and Lai Guan Ng

DOI: 10.1084/jem.20160800