Video 1.

Monocyte margination in the pulmonary vasculature. Time-lapse image sequence of maximum intensity projection displaying the behavior of GFP⁺ cells in the lungs (alveolar space) of Cx3cr1^gfp/+ mice. TRITC-dextran (250 µg) was administered i.v. to delineate the lung vasculature. Shown are the motility patterns of GFP⁺ monocytes at baseline and after treatment with LPS (10 ng), AMD3100 (5 mg/kg), or LPS and AMD3100 together. For mice treated with LPS, monocytes are observed to reduce their speed and increase duration of adherence in the lung endothelium. This phenomenon was abolished when mice were pretreated with AMD3100. Videos are representative of 3 mice per treatment. Elapsed time is shown as hours:minutes:seconds. Scale bar: 50 µm.

CXCR4 identifies transitional bone marrow premonocytes that replenish the mature monocyte pool for peripheral responses

Shu Zhen Chong, Maximilien Evrard, Sapna Devi, Jinmiao Chen, Jyue Yuan Lim, Peter See, Yiru Zhang, José M. Adrover, Bernett Lee, Leonard Tan, Jackson L.Y. Li, Ka Hang Liong, Cindy Phua, Akhila Balachander, Adrian Boey, David Liebl, Suet Mien Tan, Jerry K.Y. Chan, Karl Balabanian, John E. Harris, Mariaelvy Bianchini, Christian Weber, Johan Duchene, Josephine Lum, Michael Poidinger, Qingfeng Chen, Laurent Rénia, Cheng-I Wang, Anis Larbi, Gwendalyn J. Randolph, Wolfgang Weninger, Mark R. Looney, Matthew F. Krummel, Subhra K. Biswas, Florent Ginhoux, Andrés Hidalgo, Françoise Bachelerie, and Lai Guan Ng

DOI: 10.1084/jem.20160800