Video 1.

Smc3^fl/+ BM nucleolar 3D reconstruction. Nucleolar stain of Smc3^fl/+ BM reveals a solitary intranuclear nucleolus. ACK-lysed BM was stained with TOTAL-NUCLEAR-ID fluorescent reagents, allowing simultaneous staining of both the nucleoli (green) and total nucleus (red). Images were taken using a TCS SP5 confocal microscope (Leica) with a 63× 1.4 NA oil objective and deconvolved using AutoDeblur (Media Cybernetics). Three-dimensional rendering was performed using Imaris.

Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis

Aaron D. Viny, Christopher J. Ott, Barbara Spitzer, Martin Rivas, Cem Meydan, Efthymia Papalexi, Dana Yelin, Kaitlyn Shank, Jaime Reyes, April Chiu, Yevgeniy Romin, Vitaly Boyko, Swapna Thota, Jaroslaw P. Maciejewski, Ari Melnick, James E. Bradner, and Ross L. Levine

DOI: 10.1084/jem.20151317