Time-lapse microscopy of EBI2-deficient and -sufficient OC motility in vitro. OCs were photographed every 3 min for 2 h (differential interference contrast, 20×). OC movement was examined by tracking readily distinguishable OC vacuoles over time. At least four vacuoles per OC were averaged to calculate OC velocity (µm/min) and displacement (µm). Data are representative of more than three independent experiments. This video is shown at 20 frames/s. Bar, 80 µm. See also Fig. 5.
