Video 2.

Calcium imaging of live AGM cell cultures with application of WSS. Video file depicts sparks of intracellular calcium in individual cells after initiation of WSS (10th frame of the video). Processing of cells and images was conducted in parallel with Video 1 for static culture.

Biomechanical forces promote blood development through prostaglandin E2 and the cAMP–PKA signaling axis

Miguel F. Diaz, Nan Li, Hyun Jung Lee, Luigi Adamo, Siobahn M. Evans, Hannah E. Willey, Natasha Arora, Yu-suke Torisawa, Dwayne A. Vickers, Samantha A. Morris, Olaia Naveiras, Shashi K. Murthy, Donald E. Ingber, George Q. Daley, Guillermo García-Cardeña, and Pamela L. Wenzel

DOI: 10.1084/jem.20142235