Intravital two-photon imaging of the interaction between NK and DC in the LN draining site of uveitogenic immunization. (A) WT NK cells sorted from actin-GFP reporter mice (green) and CMTMR-labeled NK cells sorted from CXCR3−/− mice (red) were co-injected intravenously at a 1:1 ratio into EAU-immunized CD11cYFP recipients. After 24 h, NK cell DC interactions were imaged in the popliteal LN of an anesthetized mouse using 2P-IVM for 60 min. NK–DC interaction was defined as a sustained decrease in velocity on the part of the NK cell upon contact with a specific DC. (B) Green tracks indicate the path of WT NK cells and red tracks indicate the path of CXCR3−/− NK cells. Zoom from x, y, z = 213 µm, 213 µm, 64 µm; merge of z-stack), time-lapse over 60 min (10 frames/s).
