Lymph flow through the PLN. Serial fluorescent reconstruction of a naive C57BL/6 PLN. Mouse was adoptively transferred with labeled B cells and T cells 24 h before imaging. The PLN was in vivo fluorescently labeled with a–Lyve-1 and a-CD169. B cell follicles (red), T cell cortex (green), and medulla (pink) are represented with isosurfacing to aid compartment visualization (blue, subcapsular sinus). Video is animated to highlight relevant regions within the PLN and display a hypothesized model of lymph flow and antigen filtration.
