LNDCs infiltrate the medulla after UV-PR8 vaccination. A CD11c-eYFP mouse was anesthetized, and the PLN was exposed for intravital imaging. UV-PR8 was injected with a red tracer at t = 0, and 150-µm stacks were captured every 3 min for 45 min. LNDCs (green) are shown arriving in the medulla (red) underneath the outer capsule (blue). The medulla was in vivo labeled with a-f4/80-A633. Representative of four independent videos.
