LNDC migration in response to influenza vaccination. A CD11c-eYFP mouse was anesthetized, and the PLN was exposed for intravital imaging. UV-PR8 was injected with a red tracer at t = 0, and 150-µm stacks were captured every 3 min for 45 min. LNDCs (green) are shown moving toward the collagen capsule (blue), with two individual DCs highlighted (bounding boxes and tracks shown in red). Representative of four independent videos.