Dynamic nature of the microtubule plus-end in WT, but not Dock5−/−, BMMCs. BMMCs expressing GFP-tagged c-APC were sensitized with anti-DNP IgE antibody and then stimulated with DNP-HSA. Images were taken at 2-s intervals for 100 s, and a 10× time-lapse video is shown for WT and Dock5−/− BMMCs. Images are representative of 18 (for WT) or 20 (Dock5−/−) BMMCs collected from ten independent experiments. This video is shown at 5 frames/s. Bar, 5 µm.
