Animation of confocal imaging of 150-µm-thick tissue sections which shows that S. flexneri is found intracellularly in B lymphocytes within ILFs. The animation moves through human colonic tissue after ex vivo infection by WT bacteria orthogonally. Bacteria were stained with an antibody specific for S. flexneri 5a (red) and B cells with anti-CD20cy (green). DAPI nuclei staining is shown in blue.
