Time-lapse analysis of PC degranulation after IFN-γ exposure using Zinpyr-1 staining. In small intestinal organoids, PC granules were labeled with the fluorescent dye ZP-1. Movies were acquired with 15-min image intervals (days:hours:minutes:seconds). Confocal signals of single Z-planes are shown that were overlaid to bright field images. High dose IFN-γ treatment (20 ng/ml) causes a more rapid discharging of PCs (within 8 h).
