Time-lapse analysis of PC degranulation after IFN-γ exposure using Zinpyr-1 staining. In small intestinal organoids, PC granules were labeled with the fluorescent dye ZP-1. Movies were acquired with 15-min image intervals (days:hours:minutes:seconds). Confocal signals of single z-planes are shown that were overlaid to bright field images. Addition of 5 ng/ml IFN-γ causes progressive but complete PC degranulation within 12 h. Note that individual PCs extrude all granules into the crypt lumen in an all or nothing fashion.
