The +9.5 element is required for HSC genesis in the AGM. Whole-mount three-dimensional confocal imaging of +9.5−/− embryos at E10.5 with c-Kit (green) and CD31 (magenta) antibodies. Three-dimensional reconstructions were generated from z-stacks (100–120 optional sections) and the video is displayed at 5 frames per second. Related to Fig. 2.
