Crawling neutrophils shortly after DVT induction. Neutrophils (green, labeled with an anti–Ly6G-FITC antibody) crawling along the venous vessel wall (red) 2 h after the flow restricting procedure (video still in Fig. 3 A). Tracking of a 4D reconstruction of images was acquired by intravital time lapse two-photon microscopy (acquisition rate: 3 frames/min; frame rate: 8.11 frames/s).