Development of an inflammatory response in a TNF-stimulated tissue. The video shows a cremasteric venule (∼50 µm diam) of an αSMA-RFPcherry x Lys-EGFP-ki mouse (exhibiting both RFPcherry-labeled pericytes (red) and EGFP-labeled leukocytes (green), immunostained in vivo for EC junctions with Alexa Fluor-647–labeled anti–PECAM-1 mAb 390 (blue) and stimulated with TNF (300 ng/mouse i.s.). The video (captured at ∼40×) shows the development of an inflammatory response (from 2 h after injection of the cytokine), as seen from the luminal side. Images were captured at one stack per minute and the sequence shows a 120-min period. Still images are shown in Fig. 1 B.
