Luminally accessible Ecad at sites of cell extrusion. This video is related to Fig. S4. A vibratome section of an intestinal villus was stained before permeabilization for accessible hEcad (green) and after permeabilization for ZO-1 (red) and nuclei (blue). The video shows a gap after cell extrusion surrounded by accessible Ecad on the lateral side of an intestinal villus. Images were acquired and assembled as described for Video 3.
