Ezrin-WT gates the dynamics and fusion of microclusters. DT40 B cells expressing Ezrin-WT were settled onto planar lipid bilayers containing antigen (Ag) and visualized by TIRFM. Right panels show magnified images of the region indicated by the white box of Ezrin (top) and antigen (bottom) pseudocolored to aid visualization. Regions of antigen are highlighted in ezrin images by the black circles. Images were acquired every 10 s and are played back at 5 frames/s.
