T cell migration and interaction behavior within MVA-induced BALT 24 h after i.t. transfer of Ag-DCs. 24 h after i.t. transfer of SIINFEKL-loaded EGFP+ DCs (green) into MVA-treated WT recipients, many TAMRA+ OT-I T cells (red) exhibit a confined migration behavior within BALT, forming long-lasting contacts with Ag-DCs (middle). In contrast, CMAC+ polyclonal WT control T cells (cyan) display a higher motility (right). (left) Three-color overlay. Maximum intensity projection of a 132 × 132 × 54 µm three-dimensional imaging volume (six Z-slices) with a 300× time lapse.
