Video 7.

Recruitment of PLCγ2-GFP to antigen microclusters in PLCγ2-KO–reconstituted DT40 B cells.TIRFM images were collected, after interaction of cells with a bilayer containing anti-IgM as antigen, every 3 s and reconstructed at 15 frames/s.

Phospholipase C-γ2 and Vav cooperate within signaling microclusters to propagate B cell spreading in response to membrane-bound antigen

Michele Weber , Bebhinn Treanor , David Depoil , Hisaaki Shinohara , Naomi E. Harwood , Masaki Hikida , Tomohiro Kurosaki , and Facundo D. Batista

DOI: 10.1084/jem.20072619