Control and PI3Kγ-/- T cell migration in PLNs. T cells were purified from lymph nodes and spleens of control and PI3Kγ-deficient mice, labeled with CMTMR and CFSE, respectively, and injected into wild-type recipients 20 h before imaging. Second harmonic signals from collagen fibers are shown in blue. Time in minutes and seconds. Bar, 30 µm.
